Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit.
Identifieur interne : 002211 ( Main/Exploration ); précédent : 002210; suivant : 002212Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit.
Auteurs : David J. Vance [États-Unis] ; Nicholas J. MantisSource :
- Toxicon : official journal of the International Society on Toxinology [ 1879-3150 ] ; 2012.
Descripteurs français
- KwdFr :
- Anticorps monoclonaux (métabolisme), Banque de peptides, Domaine catalytique (génétique), Données de séquences moléculaires, Déterminants antigéniques des lymphocytes B (génétique), Déterminants antigéniques des lymphocytes B (métabolisme), Modèles moléculaires, Ricine (génétique), Structure secondaire des protéines (génétique), Séquence d'acides aminés.
- MESH :
- génétique : Domaine catalytique, Déterminants antigéniques des lymphocytes B, Ricine, Structure secondaire des protéines.
- métabolisme : Anticorps monoclonaux, Déterminants antigéniques des lymphocytes B.
- Banque de peptides, Données de séquences moléculaires, Modèles moléculaires, Séquence d'acides aminés.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Epitopes, B-Lymphocyte, Ricin.
- chemical , metabolism : Antibodies, Monoclonal, Epitopes, B-Lymphocyte.
- genetics : Catalytic Domain, Protein Structure, Secondary.
- Amino Acid Sequence, Models, Molecular, Molecular Sequence Data, Peptide Library.
Abstract
Residues Y₉₁-T₁₁₆ of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs), PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q₉₈, E₁₀₂, T₁₀₅, and H₁₀₆ are central to mAb recognition.
DOI: 10.1016/j.toxicon.2012.06.014
PubMed: 22750533
Affiliations:
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Le document en format XML
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Vance</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, NY 12208</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Mantis, Nicholas J" sort="Mantis, Nicholas J" uniqKey="Mantis N" first="Nicholas J" last="Mantis">Nicholas J. Mantis</name></author></analytic><series><title level="j">Toxicon : official journal of the International Society on Toxinology</title><idno type="eISSN">1879-3150</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibodies, Monoclonal (metabolism)</term><term>Catalytic Domain (genetics)</term><term>Epitopes, B-Lymphocyte (genetics)</term><term>Epitopes, B-Lymphocyte (metabolism)</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Protein Structure, Secondary (genetics)</term><term>Ricin (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps monoclonaux (métabolisme)</term><term>Banque de peptides</term><term>Domaine catalytique (génétique)</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes B (génétique)</term><term>Déterminants antigéniques des lymphocytes B (métabolisme)</term><term>Modèles moléculaires</term><term>Ricine (génétique)</term><term>Structure secondaire des protéines (génétique)</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Epitopes, B-Lymphocyte</term><term>Ricin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Epitopes, B-Lymphocyte</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Catalytic Domain</term><term>Protein Structure, Secondary</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Domaine catalytique</term><term>Déterminants antigéniques des lymphocytes B</term><term>Ricine</term><term>Structure secondaire des protéines</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Déterminants antigéniques des lymphocytes B</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Peptide Library</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Modèles moléculaires</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Residues Y₉₁-T₁₁₆ of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs), PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q₉₈, E₁₀₂, T₁₀₅, and H₁₀₆ are central to mAb recognition.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Mantis, Nicholas J" sort="Mantis, Nicholas J" uniqKey="Mantis N" first="Nicholas J" last="Mantis">Nicholas J. Mantis</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Vance, David J" sort="Vance, David J" uniqKey="Vance D" first="David J" last="Vance">David J. Vance</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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